IN VITRO EVALUATION OF LEAD REMOVAL IN WASTEWATER BY Photobacterium damselae
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Published:
2017-09-01
Keywords:
Photobacterium damselae, removal, bioremediation, lead, wastewaters.
Main Article Content
Abstract
In order to mitigate the environmental impacts caused by lead in wastewater from sectors such as: mining, petrochemical,
metallurgical and others, an in vitro evaluation of lead removal using Photobacterium damselae was carried
out. Considering isolation and biostimulation phase, obtained isolate was subjected to a selection process in a modified
culture medium, to which concentrations of 20 and 100 ppm of Pb were added, finally obtaining the pure strain
that showed resistance and /or tolerance to Pb. To determinate the remotionÂŽs capacity of Pb in wastewater two
conditions were observed: incubation at controlled temperature (25C) and incubation at room temperature in Quito-
Ecuador (southern zone at 2800msnm). Biochemical characterization of the bacteria was performed using the GN-ID
A + B Microgen Kit. In the development of bacterial growth kinetics curves and Pb removal curves, turbidimetry and
atomic absorption techniques were used, it was noted that Photobacterium damselae presented a greater rate of growth
to a maximum of 72 hours and a concentration of 20 ppm in incubation at room temperature achieving a removal rate
of 69% of the lead in the medium. From this information, the potential of this bacterium is inferred and opportunities
are opened to continue studies in the future.
metallurgical and others, an in vitro evaluation of lead removal using Photobacterium damselae was carried
out. Considering isolation and biostimulation phase, obtained isolate was subjected to a selection process in a modified
culture medium, to which concentrations of 20 and 100 ppm of Pb were added, finally obtaining the pure strain
that showed resistance and /or tolerance to Pb. To determinate the remotionÂŽs capacity of Pb in wastewater two
conditions were observed: incubation at controlled temperature (25C) and incubation at room temperature in Quito-
Ecuador (southern zone at 2800msnm). Biochemical characterization of the bacteria was performed using the GN-ID
A + B Microgen Kit. In the development of bacterial growth kinetics curves and Pb removal curves, turbidimetry and
atomic absorption techniques were used, it was noted that Photobacterium damselae presented a greater rate of growth
to a maximum of 72 hours and a concentration of 20 ppm in incubation at room temperature achieving a removal rate
of 69% of the lead in the medium. From this information, the potential of this bacterium is inferred and opportunities
are opened to continue studies in the future.
Article Details
Section
Scientific Article
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References
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Coccini, T., Caloni, F., Ramírez Cando, L. J., & De Simone, U. (2016). Cytotoxicity and proliferative capacity impairment induced on human brain cell cultures after short- and long-term exposure to magnetite nanoparticles. Journal of Applied Toxicology, (May). http://doi.org/10.1002/jat.3367
Eróstegui Revilla, C. P. (2009). Contaminación por metales pesados. Revista Científica Ciencia Médica SCEM. Romero, Karla.
Greenpeace. (2008). Informe de Contaminación en España. Barcelona.
Guevara, M. F., & Ramírez, L. J. (2015). Eichhornia crassipes, su invasividad y potencial fitorremediador. Revista de Ciencias de La Vida: La Granja, 22(2), 5–11. http://doi.org/10.17163/lgr.n22.2015.01
Hasan, S. H., Srivastava, P., & Talat, M. (2010). Biosorption of lead using immobilized Aeromonas hydrophila biomass in up flow column system: Factorial design for process optimization. Journal of Hazardous Materials, 177(1–3), 312–322. http://doi.org/10.1016/j.jhazmat.2009.12.034
Naik, M. M., & Dubey, S. K. (2013). Lead resistant bacteria: Lead resistance mechanisms, their applications in lead bioremediation and biomonitoring. Ecotoxicology and Environmental Safety, 98, 1–7. http://doi.org/10.1016/j.ecoenv.2013.09.039
Paniagua GL, Monroy E, Perches M, Negrete E, García O, V. S. (2006). Antibiotic and heavy metal resistance of Aeromonas hydrophila isolated from charal ( Chirostoma humboldtianum , Valenciannes , 1835 ). Hidrobiológia, 16(1), 75–79.
Pongratz, R., & Heumann, K. G. (1999). Production of methylated mercury, lead, and cadmium by marine bacteria as a significant natural source for atmospheric heavy metals in polar regions. Chemosphere, 39(1), 89–102. http://doi.org/10.1016/S0045-6535(98)00591-8
Ramirez, Lenin; Coba, P. (2012). AISLAMIENTO, CARACTERIZACIÓN Y CONSERVACIÓN DE BACTERIAS NO ENTÉRICAS CON CAPACIDAD DE ADAPTACIÓN EN ALTAS CONCENTRACIONES DE PLATA, PRESENTES EN UNA LAGUNA DE SEDIMENTACIÓN DE LA PLANTA MINERA DEL SECTOR EL PACHE-PORTOVELO-EL ORO. UNIVERSIDAD POLITÉCNICA SALESIANA.
Ramírez, L. (2015). Magnetite (Fe3 O4 ) nanoparticles: Are they really safe? La Granja, 21(1), 77–83. http://doi.org/10.17163/lgr.n21.2015.07
Sánchez, J., & Rodríguez, J. (2010). Fundamentos y Aspectos Microbiológicos: Biorremediación. Universidad de Oviedo, 1, 12–16.
Soto, C., Gutiérrez, S., Rey León, A., & González, E. (2010). Biotransformación de metales pesados presentes en lodos ribereños de los ríos Bogotá y Tunjuelo. Nova, 195–205.
Zorrilla, I., Chabrillón, M., Arijo, S., Díaz-Rosales, P., Martínez-Manzanares, E., Balebona, M. C., & Moriñigo, M. A. (2003). Bacteria recovered from diseased cultured gilthead sea bream (Sparus aurata L.) in southwestern Spain. Aquaculture, 218(1–4), 11–20. http://doi.org/10.1016/S0044-8486(02)00309-5